images anti phospho ser antibody identification

For example, Akt, a kinase that regulates cell survival, is activated by phosphorylation at Ser, a site surrounded by phenylalanine and tyrosine 3. Associated products. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals. Yaffe, M. Continue Continue.

  • CST Phospho(Ser/Thr) Kinase Substrate Antibody Sampler Kit
  • pSer Antibody (16B4) SCBT Santa Cruz Biotechnology
  • AntiPhospho (Ser/Thr) antibody (ab) Abcam
  • CST Phospho(Ser/Thr) Phe Antibody

  • products Compare Anti-ser Antibody Products from leading suppliers on Biocompare. View specifications Anti-phospho-Ser/Thr-Pro MPM-2 Antibody.

    CST Phospho(Ser/Thr) Kinase Substrate Antibody Sampler Kit

    In order to identify a specific kinase for a serine-proline motif in the protein that I .com/phosphothreonine-proline--phosphoserine-proline-antibody-abhtml) a variety of phospho-Ser/Thr-Pro epitopes including those on MAP2, HSP Rabbit polyclonal Phospho - (Ser/Thr) antibody. Validated in ELISA, Dot and tested in Species independent. Cited in 1 publication(s). Independently reviewed in.
    Incubate at room temperature for 30 min, on gentle shaker.

    Product Size. Buffers and proteins which contain phosphate should be avoided with this antibody. YES NO. Submit a review Submit a question. Sample Analysis Proceed to one of the following specific set of steps.

    images anti phospho ser antibody identification
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    Aspirate media from cultures; wash cells with 1X PBS; aspirate. Preparing Cell Lysates Aspirate media. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Your name Your email.

    p-Ser Antibody (16B4) is a monoclonal detecting a broad range of for detection of a broad range of serine-phosphorylated proteins in crude cell extracts, recombinant alternative to conventional secondary anti-mouse IgG reagents.

    pSer Antibody (16B4) SCBT Santa Cruz Biotechnology

    Contact. Antibody Sampler Kit for studying Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit in the Motif Anti-rabbit IgG, HRP-linked Antibodyµl. WB. Phospho-(Ser/Thr) Phe Antibody detects phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine.
    Send me a copy of this email. Dalby, K. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like The immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE.

    Scrape cells off the plate and transfer to microcentrifuge tubes. Get resources and offers direct to your inbox Sign up. Treat cells by adding fresh media containing regulator for desired time.

    images anti phospho ser antibody identification
    Anti phospho ser antibody identification
    Antibodies are purified by protein A and peptide affinity chromatography.

    Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube.

    AntiPhospho (Ser/Thr) antibody (ab) Abcam

    Continue Continue. From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Western Blotting Application Solutions Kit.

    images anti phospho ser antibody identification

    If the peptide does not bind or absorb, try other buffers in the pH 4—8 range.

    Small volumes of anti-Phospho-Ser/Thr/Tyr antibody vial(s) may occasionally use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded.

    Detect phospho-Ser/Thr-Pro MPM-2 also known as Mitotic protein #2 with Anti- phospho-Ser/Thr-Pro MPM-2 Antibody (Mouse Monoclonal Antibody), that has.

    CST Phospho(Ser/Thr) Phe Antibody

    Anti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been.
    Nonfat Dry Milk : Protein Blotting A general protocol for sample preparation.

    You would purchase the antibody at full price, test it and submit an Abreview with your data positive or negative. Blotting Membrane and Paper : This protocol has been optimized for nitrocellulose membranes.

    images anti phospho ser antibody identification

    Analyze sample by western blot see Western Immunoblotting Protocol. No data images are currently available. Signal Transduction Metabolism Amino Acids.

    images anti phospho ser antibody identification
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    Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube.

    Nonfat Dry Milk Reviews 4. Datasheet References 1 Protocols Overview. A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins.

    images anti phospho ser antibody identification

    Electrotransfer to nitrocellulose membrane Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Nitrocellulose Sandwiches Reviews 4.

    Video: Anti phospho ser antibody identification Blood Banking: Antibody Identification Panel Part 1

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