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J Exp Med. Microparticles for the delivery of DNA vaccines. In the electroporation group, three of five mice achieved complete tumor regression by Day 27 with no evidence of palpable tumor. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. The duration of luminescence acquisition was seconds. Mol Ther. All mice in these groups had to be euthanized between days 27—30 due to the bulky size of the tumor.


  • Federico A. Moreno (born April 10, ) is an American lawyer and Judge. He is a United States District Judge of the United States District Court for the. Federico A.

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    Moreno is an Article III federal judge for the United States District Court for the Southern District of Florida. He joined the court in after being. I spent some time talking with Chief Judge Moreno recently and found myself repeatedly drawn back to this idea—that passion and love for what you do can.
    Saunders1 T.

    VRC Study Team. Gene gun mediated DNA vaccination DNA-coated gold particles were delivered to the shaved abdominal region of mice using a helium-driven gene gun BioRad, Hercules, CA with a discharge pressure of psi. Figure 2. Electroporation and gene gun-mediated particle delivery are leading methods of DNA vaccine delivery that can generate protective and therapeutic levels of immune responses in experimental models. At all time points, this difference in intensity was one to two-log higher in the electroporation group as compared to the intramuscular group.

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    Mennuni federico moreno
    Muscular gene transfer using nonviral vectors. Mice were injected in the tibialis muscle of the shaved hind leg with activation of the pulse generator as instructed by the manufacturer and as previously described.

    Roy MJ, et al.

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    Needle intramuscular mediated DNA vaccination For intramuscular IM injections, the tibialis muscle was shaved and exposed in a similar manner to electroporation vaccination.

    Mol Ther.

    Marco Mennuni | Read 64 publications, and contact Marco Mennuni on ResearchGate, the professional network for scientists. Stefan-van Staden, Rahel Girmai Bokretsion, Jacobus Frederick van Staden, Fernando Navarro-Villoslada, María C.

    Moreno-Bondi, Guillermo Orellana. Mauro Giustini, Mattia Autullo, Mauro Mennuni, Gerardo Palazzo, Antonia Mallardi. Maurizio Carlini, Sonia Castellucci, Andrea Mennuni.

    Federico Galli, Luca Bonfanti, Sofia Capelli, Flavio Manenti, Claudia L. Bianchi, Gregory S.

    Patience. Abel Zúñiga-Moreno, Carmen M. Reza-San Germán, Sergio O. Flores-Valle.
    District Judge Federico Moreno". John R. Safety and immunogenicity of a bivalent cytomegalovirus DNA vaccine in healthy adult subjects. Languages Add links. Antigen presentation and T cell stimulation by dendritic cells. Morales is a prominent transactional attorney in Miami.

    In the electroporation group, three of five mice achieved complete tumor regression by Day 27 with no evidence of palpable tumor.

    images mennuni federico moreno
    Mennuni federico moreno
    Nat Biotechnol.

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    Views Read Edit View history. Paavonen J. In addition, gene expression can be detected in as little as one hour after DNA administration and can be sustained as long as 28 days with electroporation mediated gene delivery as compared to intramuscular administration.

    By demonstrating the activity of the vaccine candidate at a relatively low dose level that can be extrapolated into larger species, we are also well positioned to translate these findings into the clinical setting. Moreno served as a judge on the Dade County Court from to All animal procedures were performed according to approved protocols and in accordance with the recommendations for the proper use and care of laboratory animals.

    Federico Kennedy · Federico Knez · Federico Lalatta costerbosa · Federico Federico Mennuni, cfa · Federico Mentegari · Federico Mentegazzi · Federico Mestrone msc, ceng · Federico Monteporzi · Federico Mor · Federico Moreno sancho.

    Federico Gessi · Federico Geuna · Federico Ghelfi · Federico Gherardi · Federico Gheza · Federico Ghezzi · Federico Ghiani · Federico Ghidelli · Federico. Cancer Institute-Frederick Animal Production Area (Frederick, Maryland) and kept in . Cipriani B, Fridman A, Bendtsen C, Dharmapuri S, Mennuni C, Pak I, et al. Roos AK, Moreno S, Leder C, Pavlenko M, King A, Pisa P.

    Enhancement of .
    Markedly enhanced immunogenicity of a Pfs25 DNA-based malaria transmission-blocking vaccine by in vivo electroporation. Three previous studies in which electroporation and gene gun were compared varied the number of administration sites and used 16 to times the amount of DNA vaccine dose for the electroporation groups. The serum chemiluminescent reaction was measured in triplicate for each mouse. Address correspondence to Sara I.

    These results indicate that delivery of the luciferase plasmid by electroporation results in a higher efficacy in gene transfer as compared to conventional intramuscular injection.

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    Three mice were assigned to each group, including a control group which received no plasmid.

    Enhanced T-cell immunogenicity of plasmid DNA vaccines boosted by recombinant modified vaccinia virus Ankara in humans.

    Safety and immunogenicity of a bivalent cytomegalovirus DNA vaccine in healthy adult subjects. Both electroporation EP and intramuscular IM injections were performed in the left hind leg, with control PBS injections in the right hind leg.

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    Needle intramuscular mediated DNA vaccination For intramuscular IM injections, the tibialis muscle was shaved and exposed in a similar manner to electroporation vaccination. Cancer vaccines. With regards to antigen expression, we and others have demonstrated that electroporation based DNA intramuscular delivery results in higher and more persistent levels of protein expression using SEAP and luciferase.

    Comments (3)

    1. Bagami

      Reply

      For intramuscular IM injections, the tibialis muscle was shaved and exposed in a similar manner to electroporation vaccination.

    2. Magami

      Reply

      Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa.